Journal: Nucleic Acids Research

Article Title: MLGA—a rapid and cost-efficient assay for gene copy-number analysis

doi: 10.1093/nar/gkm651

Figure Lengend Snippet: ( a ) Multiplex ligation-dependent genome amplification (MLGA), reaction scheme. (I) Genomic DNA is digested by restriction enzyme to generate targets with defined ends. (II) Each MLGA probe consists of two oligonucleotides, one selector oligo of 70–74 nt (green) and one general vector oligo of 34 nt (red). MLGA probe together with DNA-ligase forms circular DNA of target molecules after denaturation and hybridization. (III) To reduce background signal in the assay, undesirable, linear DNA is degraded by exonuclease I (Exo I). (IV) Multiplex PCR is facilitated by using universal primers that hybridize to a sequence in the vector. PCR products are analyzed using the Agilent Bioanalyzer 2100™ electrophoresis system. ( b ) Data from an MLGA set of 14 probes targeting loci on human chromosomes 13, 18, 21, X and Y. The upper graph shows the resulting elution diagrams from analyses of male and female DNA pools.

Article Snippet: Pooled samples of male and female DNA from Promega (cat# G147A 20745001, cat# G152 20215001) were used as references.

Techniques: Multiplex Assay, Ligation, Amplification, Plasmid Preparation, Hybridization, Sequencing, Electrophoresis

Journal: Cancer cell

Article Title: Comprehensive Molecular Characterization Identifies Distinct Genomic and Immune Hallmarks of Renal Medullary Carcinoma

doi: 10.1016/j.ccell.2020.04.002

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Control DNA was used from Promega (Human Genomic DNA Female N 30742202/Male N 30993901).

Techniques: Control, Virus, Recombinant, Library Quantification, DNA Methylation Assay, Empire Assay, Imaging, Sequencing, Plasmid Preparation, Software